| Unlockable: | How to Unlock: |
| All story modes | Complete the game in both storylines. |
| Harada Sanosuke | Complete Chapter 5 to unlock Harada Sanosuke in two player mode. |
| Hijikata Toshizo | Complete Hijikata Toshizo's story mode quest to unlock him in two player mode. |
| Hirayama Gorou | Kill Hirayama Gorou at the end of Chapter 1 to unlock him in two player mode. |
| Imperial character story modes | Complete the game in the Imperial storyline. |
| Itou Kashiitarou | Kill Itou Kashiitarou at the end of Chapter 4 to unlock him two player mode. |
| Katsura Kogoro | Complete Katsura Kogoro's story mode quest to unlock him in two player mode. |
| Kondo Isami | Complete Kondo Isami's story mode quest to unlock him in two player mode. |
| Nagakura Shinpachi | Complete Chapter 5 to unlock him in two player mode. |
| Okita Souji | Complete Okita Souji's story mode quest to unlock him in two player mode. |
| Saigo Takamori | Complete Saigo Takamori's story mode quest to unlock him in two player mode. |
| Saito Hajime | Complete Saito Hajime repeatedly on Te-awase to unlock him in two player mode. |
| Sakamato Ryoma | Complete Sakamato Ryoma's story mode quest to unlock him in two player mode. |
| Serizawa Kamo | Complete Serizawa Kamo's side quest to unlock him in two player mode. |
| Shinsengumi character Story modes | Complete the game in the Shinsengumi storyline. |
| Shinsengumi Lower Class member | Start a game on the Shinsengumi storyline to unlock this character in two player mode. |
| Takeda Kanryuusai | Complete the mission with Takeda Kanryuusai at Masuya to unlock him in two player mode. |
| Tetsunosuke Ichimura | Meet Tetsunosuke Ichimura during Chapter 6 of the Shinsengumi storyline to unlock in two player mode. |
| Toudou Heisuke | Kill Toudou Heisuke at the end of Chapter 4 to unlock him in two player mode. |
| Two Hitokiri | Complete the story mode for Two Hitokiri to unlock him in two player mode. |
| Yamanami Keisuke | Kill Yamanami Keisuke at the end of Chapter 3 to unlock him in two player mode. |
| Yamazaki Susumu | Start a game in the Shinsengumi storyline to unlock him in two player mode. |
| Unlockable: | How to Unlock: |
| Choshu Ryu | In the Imperial storyline, get all techniques of the school then defeat the sword master. |
| Edo Ryu | In the Imperial storyline, get all techniques of the school then defeat the sword master. |
| Hokushin Ittou Ryu | In either storyline, get all techniques of the school then defeat the sword master. |
| Satsuma Ryu (Jigen Ryu) | In the Imperial storyline, get all techniques of the school then defeat the sword master. |
| Shintou Munen Ryu | In either storyline, get all techniques of the school then defeat the sword master. |
| Spear Ryu (Houzouin Ryu) | In the Shinsengumi storyline, get all techniques of the school then defeat the spear master. |
| Tama Ryu (Tennen Rishin Ryu) | In the Shinsengumi storyline, get all techniques of the school then defeat the sword master before Chapter 6. |
